RFP 抗体 (Red Fluorescent Protein) (AA 234)

Details for Product anti-RFP Antibody No. ABIN129578, 供应商: Log in to see
抗原
抗原表位
AA 234
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适用
Discosoma
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宿主

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克隆类型
多克隆
标记
This RFP antibody is un-conjugated
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应用范围
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
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免疫原 The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant
亚型 IgG
产品特性 Concentration Definition: by UV absorbance at 280 nm
纯化方法 Pre-adsorbed
别名 RFP
背景 Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
研究领域 Tags/Labels
应用备注 Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
厂商提供的图像
Western Blotting (WB) image for anti-RFP 抗体 (Red Fluorescent Protein) (AA 234) (ABIN129578) Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. L...
有引用在: Viganò, Möbius, Götz, Dimou: "Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain." in: Nature neuroscience, Vol. 16, Issue 10, pp. 1370-2, 2013 (PubMed). (1:500).

Yoshinaga, Ohkubo, Sasaki, Nuriya, Ogawa, Yasui, Tabata, Nakajima: "A phosphatidylinositol lipids system, lamellipodin, and Ena/VASP regulate dynamic morphology of multipolar migrating cells in the developing cerebral cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 34, pp. 11643-56, 2012 (PubMed).

Becher, Hambardzumyan, Walker, Helmy, Nazarian, Albrecht, Hiner, Gall, Huse, Jabado, MacDonald, Holland: "Preclinical evaluation of radiation and perifosine in a genetically and histologically accurate model of brainstem glioma." in: Cancer research, Vol. 70, Issue 6, pp. 2548-57, 2010 (PubMed).

Fries, Reddy, Mikhaylova, Haverkamp, Wei, Ma: "Dynamic cellular translocation of caldendrin is facilitated by the Ca2+-myristoyl switch of recoverin." in: Journal of neurochemistry, Vol. 113, Issue 5, pp. 1150-62, 2010 (PubMed).

Chambers, Takimoto: "Trafficking of Sendai virus nucleocapsids is mediated by intracellular vesicles." in: PLoS ONE, Vol. 5, Issue 6, pp. e10994, 2010 (PubMed).

Shi, Wu, Liu, Tsao, Wang, Kobatake, Bentley, Payne: "Chitosan fibers: versatile platform for nickel-mediated protein assembly." in: Biomacromolecules, Vol. 9, Issue 5, pp. 1417-23, 2008 (PubMed).

Dallaire, Bellmann, Laplante, Ga: "Obese mice lacking inducible nitric oxide synthase are sensitized to the metabolic actions of peroxisome proliferator-activated receptor-gamma agonism." in: Diabetes, Vol. 57, Issue 8, pp. 1999-2011, 2008 (PubMed).

Burton, Johnson, Sato, Koh, Mulholland, Stout, Chatziioannou, Phelps, Wu, Wu: "Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer." in: Nature medicine, Vol. 14, Issue 8, pp. 882-8, 2008 (PubMed).