V5 Epitope 标记 抗体

ABIN129577 产品详细信息, 供应商: Log in to see
抗原
适用
标记
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克隆类型
多克隆
标记
非结合性
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应用范围
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Supplier
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免疫原 This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.
序列 GKPIPNPLLG LDST
亚型 IgG
产品特性 Concentration Definition: by UV absorbance at 280 nm
别名 V5-Tag
物质类 Tag
背景 Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG, HA and V5. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
基因ID 55775699
研究领域 Tags/Labels
应用备注 Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
限制 仅限研究用
状态 Liquid
浓度 0.73 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
厂商提供的图像
Western Blotting (WB) image for anti-V5 Epitope 标记 抗体 (ABIN129577) Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by west...