Immunohistochemistry on frozen sections. Immunoprecipitation. Flow Cytometry: 1:10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western Blot: 1:10 - 1/100, This antibody detects intact 50 kDa LOX-1 and also a prominent 30 kDa proteolytic fragment in western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.1 mg/mL
缓冲液
PBS, 0.09 % Sodium Azide, 1 % BSA
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
有效期
12 months
抗原
OLR1
(Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
LOX-1 is a 40-50 kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (1,4) and inflammatory diseases such as atherosclerosis (1) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs).Synonyms: C-type lectin domain family 8 member A, CLEC8A, LOX-1, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, Ox-LDL receptor 1, Ox-LDL receptor 1 soluble form, Oxidized low-density lipoprotein receptor 1, sLOX-1, sLOX1