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PAG1 抗体 (AA 97-432)

PAG1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal MEM-255 unconjugated
产品编号 ABIN120652
发货至: 中国
  • 抗原 See all PAG1 抗体
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    抗原表位
    • 16
    • 10
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 97-432
    适用
    • 28
    • 27
    • 24
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 43
    • 4
    小鼠
    克隆类型
    • 43
    • 4
    单克隆
    标记
    • 19
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PAG1 antibody is un-conjugated
    应用范围
    • 34
    • 16
    • 13
    • 13
    • 12
    • 8
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    特异性
    The antibody recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG).
    纯化方法
    Protein-A affinity chromatography
    纯度
    > 95 % pure (by SDS-PAGE)
    免疫原
    Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
    克隆位点
    MEM-255
    亚型
    IgG2a
    Top Product
    Discover our top product PAG1 Primary Antibody
  • 应用备注
    Flow Cytometry: 2 mg/mL (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.
    有效期
    12 months
  • 抗原
    PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))
    别名
    PAG1 / Csk-Binding Protein (PAG1 产品)
    别名
    Cbp antibody, F730007C19Rik antibody, Pag antibody, CBP antibody, PAG antibody, MGC80192 antibody, phosphoprotein associated with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 antibody, phosphoprotein membrane anchor with glycosphingolipid microdomains 1 L homeolog antibody, Pag1 antibody, PAG1 antibody, pag1.L antibody, pag1 antibody
    背景
    PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.Synonyms: CBP, PAG, Phosphoprotein associated with glycosphingolipid-enriched microdomains 1, Transmembrane adapter protein PAG, Transmembrane phosphoprotein Cbp
    基因ID
    55824
    UniProt
    Q9NWQ8
    途径
    p53 Pathway, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
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