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SLC9A3R1 抗体

SLC9A3R1 适用: 人 WB, IP, IHC (p) 宿主: 小鼠 Monoclonal EBP10 unconjugated
产品编号 ABIN120629
发货至: 中国
  • 抗原 See all SLC9A3R1 抗体
    SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This SLC9A3R1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    The antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
    纯化方法
    Affinity Chromatography on Protein A
    免疫原
    Bacterially produced recombinant full-length human NHERF
    克隆位点
    EBP10
    亚型
    IgG2b
    Top Product
    Discover our top product SLC9A3R1 Primary Antibody
  • 应用备注
    Immunoprecipitation. Western Blotting: 2 m g/mL, 60 min on vertical incubator. Positive control: RAJI human lymphoma cell lysate. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. 10 % separating SDS-PAGE gel. Immunohistochemistry (paraffin sections): Recommended dilution: 5 m g/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf Life: one year from despatch.
    有效期
    12 months
  • 抗原
    SLC9A3R1 (Solute Carrier Family 9, Subfamily A (NHE3, Cation Proton Antiporter 3), Member 3 Regulator 1 (SLC9A3R1))
    别名
    SLC9A3R1 / NHERF (SLC9A3R1 产品)
    别名
    EBP50 antibody, NHERF antibody, NHERF-1 antibody, NHERF1 antibody, NPHLOP2 antibody, SLC9A3R1 antibody, cb895 antibody, slc9a3r2 antibody, wu:fd20a02 antibody, zgc:55541 antibody, zgc:77629 antibody, EBP-50 antibody, NHE-RF antibody, nherf antibody, nherf-1 antibody, nherf1 antibody, slc9a3r1 antibody, SLC9A3 regulator 1 antibody, microRNA 3615 antibody, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1a antibody, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 antibody, SLC9A3 regulator 1 S homeolog antibody, SLC9A3R1 antibody, MIR3615 antibody, slc9a3r1a antibody, Slc9a3r1 antibody, slc9a3r1.S antibody
    背景
    NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.Synonyms: EBP50, Ezrin-radixin-moesin-binding phosphoprotein 50, NHERF-1, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1
    基因ID
    9368
    UniProt
    O14745
    途径
    Proton Transport, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome
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