Cytokeratin 18 抗体
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- 抗原 See all Cytokeratin 18 (KRT18) 抗体
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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适用
- 哺乳动物
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 特异性
- The antibody C-04 reacts with Cytokeratin 18 (45 kDa).
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Cytoskeleton preparation of epidermal carcinoma cell line A431
- 克隆位点
- C-04
- 亚型
- IgG1
- Top Product
- Discover our top product KRT18 Primary Antibody
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anti-Keratin 18 (KRT18) (AA 53-150) antibody
KRT18 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, FACS, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal DC-10 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 哺乳动物 WB, FACS, ELISA, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C-04 unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 1-423) antibodyKRT18 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 238-396) antibodyKRT18 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Keratin 18 (KRT18) (AA 72-380) antibodyKRT18 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) (AA 102-396) antibodyKRT18 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人, 犬, Cat WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal DE-K18 unconjugated
anti-Keratin 18 (KRT18) antibodyKRT18 适用: 人 WB, IHC, IF, FACS, StM, ISt 宿主: 兔 Monoclonal KRT18-2808R unconjugated Recombinant Antibody
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- 应用备注
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Immunohistochemistry on paraffin sections. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Western Blot: 1-2 mg/mL, overnight in 4 °C, Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer, Non-reducing conditions. SDS-PAGE (10 % separating gel). Positive control: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH ~7.4 with 15 mM Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
- 别名
- Cytokeratin 18 (KRT18 产品)
- 别名
- CK18 antibody, K18 antibody, Krt1-18 antibody, CYK18 antibody, DreK18 antibody, cb83 antibody, dapk1 antibody, sb:cb83 antibody, wu:fa13f02 antibody, wu:fb36b04 antibody, krt18 antibody, MGC64569 antibody, MGC75922 antibody, KRT18 antibody, CK-18-A antibody, K18-A antibody, NC-11 antibody, cyk18 antibody, k18 antibody, krt18-a antibody, krt18-b antibody, krt18a antibody, xkendob antibody, keratin 18 antibody, keratin 18, type I L homeolog antibody, keratin 18, type I antibody, keratin, type I cytoskeletal 18 antibody, K18, simple type I keratin antibody, Keratin, type I cytoskeletal 18 antibody, keratin 18, type I S homeolog antibody, Krt18 antibody, KRT18 antibody, krt18 antibody, krt18.L antibody, LOC700569 antibody, LOC100136778 antibody, k1c18 antibody, LOC100008885 antibody, krt18.S antibody
- 背景
- Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 18 belongs to type I family (acidic cytokeratins).Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
- 基因ID
- 3875
- UniProt
- P05783
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
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