小鼠 anti-鸭 IgY (Light Chain) Antibody
FACS, WB, EIA
Monoclonal
14A3
IgG1
unconjugated
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- 抗原 See all IgY products
- IgY
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抗原表位
- Light Chain
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适用
- 鸭
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)
- 产品特性
- Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting.
- 纯化方法
- Affinity chromatography on Protein G
- 免疫原
- Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- 克隆位点
- 14A3
- 亚型
- IgG1
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- 应用备注
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Flow Cytometry. ELISA. Western Blot.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS containing 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- 抗原
- IgY
- 别名
- IgG + IgY (IgY 产品)
- 背景
- IgY Light Chain is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y
- 分子量
- 25 kDa
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