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MADCAM1 抗体

MADCAM1 适用: 人 WB, FACS, EIA 宿主: 小鼠 Monoclonal 314G8 unconjugated
产品编号 ABIN120238
发货至: 中国
  • 抗原 See all MADCAM1 抗体
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    适用
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    宿主
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    小鼠
    克隆类型
    • 47
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    单克隆
    标记
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    This MADCAM1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    特异性
    This antibody recognises Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). MAdCAM-1 expression has also been reported in the gut lamina propria but Clone 314G8 does not recognise MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    Affinity Chromatography on Protein G.
    免疫原
    Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
    克隆位点
    314G8
    亚型
    IgG1
    Top Product
    Discover our top product MADCAM1 Primary Antibody
  • 应用备注
    ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa. Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50, This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Olloquequi, Montes, Prats, Rodríguez, Montero, García-Valero, Ferrer: "Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients." in: The European respiratory journal, Vol. 37, Issue 2, pp. 289-98, (2011) (PubMed).

    Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." in: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, (2004) (PubMed).

  • 抗原
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    别名
    MAdCAM1 (MADCAM1 产品)
    别名
    MACAM1 antibody, AV211525 antibody, mucosal vascular addressin cell adhesion molecule 1 antibody, MADCAM1 antibody, Madcam1 antibody
    背景
    MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
    基因ID
    8174
    NCBI登录号
    NP_570116
    UniProt
    Q13477
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