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MADCAM1 抗体

MADCAM1 适用: 人 WB, FACS, IHC (p), EIA, Func 宿主: 小鼠 Monoclonal 314G8 unconjugated
产品编号 ABIN120237
发货至: 中国
  • 抗原 See all MADCAM1 抗体
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This MADCAM1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    This antibody recognises Human mucosal addressin cell adhesion molecule 1 (MAdCAM-1). Clone 314G8 does not recognise MAdCAM-1 in the gut lamina propria. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.
    纯化方法
    Affinity Chromatography on Protein G
    内毒素水平
    Low Endotoxin
    免疫原
    Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
    克隆位点
    314G8
    亚型
    IgG1
    Top Product
    Discover our top product MADCAM1 Primary Antibody
  • 应用备注
    ELISA. Western Blot: this antibody detects a band of approximately 60 kDa. Functional Assays. Flow Cytometry: 1/10 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining, This product does not require antigen retrieval prior to staining of paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4 without preservatives or stabilizers
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    别名
    MAdCAM1 (MADCAM1 产品)
    别名
    MACAM1 antibody, AV211525 antibody, mucosal vascular addressin cell adhesion molecule 1 antibody, MADCAM1 antibody, Madcam1 antibody
    背景
    MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1
    基因ID
    8174
    NCBI登录号
    NP_570116
    UniProt
    Q13477
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