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His 标记 抗体

ABIN119354 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
18
3
1
宿主
小鼠
397
178
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
58
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
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6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
528
372
159
106
77
75
66
25
12
10
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
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免疫原 PAX6 transcription factor linked to Histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
序列 HHHHHH
克隆位点 AD1-1-10
亚型 IgG1
纯化方法 Affinity Chromatography on Protein G
别名 6xHistidine Epitope Tag
物质类 Tag
背景 The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
应用备注 Immunhistology on Frozen Sections. ELISA. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, containing 0.09 % Sodium Azide as a preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
背景 Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).