His 标记 抗体

ABIN119353 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
24
22
14
3
2
宿主
小鼠
434
184
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
59
45
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
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5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
554
386
166
107
99
77
69
25
13
10
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
选项
Supplier
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免疫原 PAX6 transcription factor linked to histidine tag. Remarks: Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
序列 HHHHHH
克隆位点 AD1-1-10
亚型 IgG1
纯化方法 Affinity Chromatography on Protein G
别名 6xHistidine Epitope Tag
物质类 Tag
背景 The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
应用备注 Immunhistology on Frozen Sections. ELISA. Immunoprecipitation. Western Blot (1/500-1/1000). In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS containing 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (ABIN119353) Western blot of HIS-tagged protein probed with mouse anti histidine tag (ABIN119353) ...
背景 De Vooght, Caljon, Stijlemans, De Baetselier, Coosemans, Van den Abbeele: "Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly." in: Microbial cell factories, Vol. 11, pp. 23, 2012 (PubMed).

Diefenbacher, Thorsteinsdottir, Spang: "The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 25027-38, 2011 (PubMed).

Alvarez, López-Pacheco, Aguilar-Yañez, Portillo-Lara, Mendoza-Ochoa, García-Echauri, Freiden, Schultz-Cherry, Zertuche-Guerra, Bulnes-Abundis, Salgado-Gallegos, Elizondo-Montemayor, Hernández-Torre: "Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method." in: PLoS ONE, Vol. 5, Issue 4, pp. e10176, 2010 (PubMed).

Gunnarsen, Lunde, Kristiansen, Bogen, Sandlie, Løset: "Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA." in: BMC biotechnology, Vol. 10, pp. 8, 2010 (PubMed).

Wrighton, Lin, Yu, Feng: "Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9755-63, 2009 (PubMed).

Bahi, Dreyer: "Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization." in: Genes, brain, and behavior, Vol. 7, Issue 2, pp. 244-56, 2008 (PubMed).

Hwang, Duvall, Tomlinson, Boackle et al.: "Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable ..." in: Molecular immunology, Vol. 45, Issue 9, pp. 2570-80, 2008 (PubMed).

Bahi, Kusnecov, Dreyer: "The role of tissue-type plasminogen activator system in amphetamine-induced conditional place preference extinction and reinstatement." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 33, Issue 11, pp. 2726-34, 2008 (PubMed).

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008 (PubMed).

Hoffmann, Schellack, Textor, Konold, Schmitz, Cerwenka, Pflanz, Watzl: "Identification of CLEC12B, an inhibitory receptor on myeloid cells." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22370-5, 2007 (PubMed).

Zheng, Kohler, Chen, Weber, Khan, Johnson, Orentas: "Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen." in: BMC immunology, Vol. 8, pp. 4, 2007 (PubMed).

Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

Suen, Wu, Chen, Wu, Chiang: "Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice." in: Immunology, Vol. 103, Issue 3, pp. 301-9, 2001 (PubMed).

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