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His 标记 抗体 (HRP)

ABIN119352 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
18
3
1
宿主
小鼠
397
178
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
HRP
57
44
35
15
13
11
9
8
8
7
7
7
7
7
7
7
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
528
372
160
106
77
75
66
26
12
10
9
7
7
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Available images

免疫原 PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
序列 HHHHHH
克隆位点 AD1-1-10
亚型 IgG1
纯化方法 Affinity Chromatography on Protein G
别名 6xHistidine Epitope Tag
物质类 Tag
背景 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
应用备注 ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer
储存条件 4 °C
储存方法 Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
厂商提供的图像
Western Blotting (WB) image for anti-His 标记 抗体 (HRP) (ABIN119352) Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HIS...
背景 Diefenbacher, Thorsteinsdottir, Spang: "The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 25027-38, 2011 (PubMed).

Alvarez, López-Pacheco, Aguilar-Yañez, Portillo-Lara, Mendoza-Ochoa, García-Echauri, Freiden, Schultz-Cherry, Zertuche-Guerra, Bulnes-Abundis, Salgado-Gallegos, Elizondo-Montemayor, Hernández-Torre: "Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method." in: PLoS ONE, Vol. 5, Issue 4, pp. e10176, 2010 (PubMed).

Gunnarsen, Lunde, Kristiansen, Bogen, Sandlie, Løset: "Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA." in: BMC biotechnology, Vol. 10, pp. 8, 2010 (PubMed).

Wrighton, Lin, Yu, Feng: "Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9755-63, 2009 (PubMed).

Bahi, Dreyer: "Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization." in: Genes, brain, and behavior, Vol. 7, Issue 2, pp. 244-56, 2008 (PubMed).

Hwang, Duvall, Tomlinson, Boackle et al.: "Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable ..." in: Molecular immunology, Vol. 45, Issue 9, pp. 2570-80, 2008 (PubMed).

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008 (PubMed).

Hoffmann, Schellack, Textor, Konold, Schmitz, Cerwenka, Pflanz, Watzl: "Identification of CLEC12B, an inhibitory receptor on myeloid cells." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22370-5, 2007 (PubMed).

Zheng, Kohler, Chen, Weber, Khan, Johnson, Orentas: "Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen." in: BMC immunology, Vol. 8, pp. 4, 2007 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

Suen, Wu, Chen, Wu, Chiang: "Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice." in: Immunology, Vol. 103, Issue 3, pp. 301-9, 2001 (PubMed).