His 标记 抗体 (Biotin)

ABIN119351 产品详细信息, 供应商: Log in to see
抗原
  • AKR1B11
  • AKR1B12
  • ALDRLn
  • ARL-1
  • ARL1
  • HIS
  • HSI
  • aldo-keto reductase family 1, member B10 (aldose reductase)
  • AKR1B10
适用
标记
616
24
22
14
3
2
宿主
小鼠
434
184
27
23
4
4
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
Biotin
59
44
36
15
14
12
11
10
10
10
10
9
8
8
8
8
7
7
6
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
554
386
166
107
99
77
69
25
13
10
9
7
7
6
6
6
5
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 PAX6 transcription factor linked to histidine tag
序列 HHHHHH
克隆位点 AD1-1-10
亚型 IgG1
纯化方法 Protein G affinity chromatography
别名 6xHistidine Epitope Tag
物质类 Tag
应用备注 ELISA: 1/1000. Western Blot: 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is routinely tested in ELISA using histidine tagged ubiquitin and in Western blotting using histidine tagged recombinant protein control.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS with 0.09 % sodium azide as preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Western Blotting (WB) image for anti-His Tag antibody (Biotin) (ABIN119351) Western blot of HIS tagged protein probed with mouse anti Histidine Tag - Biotin (ABI...
背景 Bahi, Boyer, Kafri, Dreyer: "CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus." in: The European journal of neuroscience, Vol. 19, Issue 6, pp. 1621-33, 2004 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

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