NEW: 请通过本地经销商订购 4A Biotech Co.,Ltd.

Keratin 16 (KRT16) (C-Term) 抗体

ABIN119041 产品详细信息, 供应商: Log in to see
抗原
  • KRT17
  • krt16
  • CK16
  • FNEPPK
  • K16
  • K1CP
  • KRT16A
  • NEPPK
  • AI324768
  • Krt1-16
  • Ka16
  • keratin 17
  • keratin 16
  • keratin 16 (focal non-epidermolytic palmoplantar keratoderma)
  • KRT17
  • KRT16
  • Krt16
  • krt16
抗原表位
C-Term
31
10
7
7
7
5
3
2
2
1
1
适用

108
28
26
2
2
2
2
2
2
1
宿主
小鼠
72
34
3
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
3
3
2
2
1
1
1
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
72
62
31
27
15
13
8
4
3
3
3
2
2
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

免疫原 Synthetic peptide from the C-terminus of human cytokeratin 16
克隆位点 LL025
亚型 IgG1
特异性 This antibody is specific to a 48 kDa protein which is identified as cytokeratin 16. Cytokeratin 16 is expressed in keratinocytes which are undergoing rapid turnover in the suprabasal region. High concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation related keratins. Cellular localization: Cytoplasmic.
交叉反应 (详细) Species reactivity (tested):Human.
纯化方法 Supernatant
别名 Cytokeratin 16 (KRT16 Antibody 摘要)
背景 Keratin 16 is expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10 % of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating. Two cytokeratins, 6 and 16, are expressed when keratincytes are undergoing rapid turnover in various pathological states, wound healing, psoriasis and some carcinomas.Synonyms: CK16, Cytokeratin-16, K16, KRT16, KRT16A, Keratin 16, Keratin type I cytoskeletal 16, Keratin-16
基因ID 3868
NCBI登录号 NP_005548
UniProt P08779
研究领域 Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
应用备注 Immunohistochemistry: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended positive control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Keratin 16 (KRT16) (C-Term) 抗体 (ABIN119041) Formalin fixed paraffin embedded human squamous carcinoma of lung stained with Cytoke...
有引用在: Hachiya, Sriwiriyanont, Fujimura, Ohuchi, Kitahara, Takema, Kitzmiller, Visscher, Tsuboi, Boissy: "Mechanistic effects of long-term ultraviolet B irradiation induce epidermal and dermal changes in human skin xenografts." in: The American journal of pathology, Vol. 174, Issue 2, pp. 401-13, 2009 (PubMed).

背景 Markey, Lane, Macdonald, Leigh: "Keratin expression in basal cell carcinomas." in: The British journal of dermatology, Vol. 126, Issue 2, pp. 154-60, 1992 (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).