Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA. Immunoprecipitation. Western Blot: 1/1000 - 1/5000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular ''nick sensor'' and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.Synonyms: ADPRT, ADPRT 1, NAD(+) ADP-ribosyltransferase 1, PARP-1, PPOL, Poly [ADP-ribose] polymerase 1, Poly[ADP-ribose] synthetase 1