山羊 anti-Cow IgG (Whole Molecule) Antibody (HRP)
WB, IHC (fro), IHC (p), EIA
Polyclonal
HRP
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- 抗原 See all IgG products
- IgG
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抗原表位
- Whole Molecule
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适用
- Cow
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 纯化方法
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Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. - 免疫原
- Bovine IgG whole molecule.
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Rabbit anti-Cow IgG (Fc Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
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Suitable for Immunoblotting (1/1,000-1/4,000, Western or dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 0.8 mg/mL
- 缓冲液
- 0.01 M Potassium Phosphate, 0.21 M Sodium Chloride, pH 7.2 containing 15 mg/mL BSA (IgG and Protease free) as stabilizer.
- 储存液
- Azide free
- 注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Synonyms: Bovine Immunoglobulin G
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