Biotin 抗体 (HRP)

Details for Product anti-Biotin Antibody No. ABIN118070, 供应商: Log in to see
抗原
适用
化学剂
265
31
4
2
1
1
1
宿主
山羊
177
83
52
克隆类型
多克隆
标记
This Biotin antibody is conjugated to HRP
40
32
29
22
19
11
9
5
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
161
118
115
78
73
34
23
17
17
14
11
10
5
4
4
3
2
2
2
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
纯化方法 Immunoaffinity chromatography
物质类 Chemical
背景 Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
研究领域 Signaling, Metabolism, Epitope Tags, Tags/Labels
应用备注 Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy andimmunohistochemistry as well as other peroxidase-antibody based enzymatic assaysrequiring lot-to-lot consistency. Recommended dilutions: Western blot 1: 500 - 1: 3,000. ELISA 1: 5,000 - 1: 25,000. Immunohistochemistry 1: 300 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL of deionized water or equivalent.
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Gentamicin Sulfate, 10 mg/mL BSA (IgG and Protease free)
储存液 Gentamicin sulfate
注意事项 This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use
储存条件 4 °C/-20 °C
储存方法 Store vial at 4 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
背景 Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).

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