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ATG7 抗体 (AA 558-569)

Name: ATG7 抗体 (AA 558-569)
SKU: ABIN118061
Availability: OutOfStock

ATG7 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated

(2 references)

产品编号 ABIN118061

发货至: 中国

Datasheet as PDF

抗原 See all ATG7 抗体

ATG7 (ATG7 Autophagy Related 7 (ATG7))
抗原表位
22

16

15

9

6

6

6

6

5

4

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 558-569
适用
125

40

28

2

1

1

1

1

1

1

1
人
宿主
113

13

2
兔
Compatible Secondaries
克隆类型
106

22
多克隆
标记
63

9

9

8

6

6

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This ATG7 antibody is un-conjugated
应用范围
107

64

47

26

18

15

14

14

13

9

4

4

3

2

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100 % homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.

纯化方法

Immunoaffinity chromatography

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.

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应用备注

This affinity purified antibody has been tested for use in ELISA (1: 15,000-1: 75,000) and byWestern blot (1: 500- 1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 78 kDa in size corresponding to human Apg7 bywestern blotting in the appropriate cell lysate or extract. Human Apg7 has been reported toform a homodimer at approximately 160 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.46 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Dilute only prior to immediate use

储存条件

-20 °C

储存方法

Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
Aoki, Kobayashi, Tanida, Hatano, Komori, Matsumoto, Nishioka, Uemura: "[A difficult case of esophageal and gastric double cancer with pleural and pericardial effusion following chemo-radiotherapy (CRT)]" in: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 34, Issue 12, pp. 1979-81, (2008) (PubMed).

Fu, Yuan, Sun, Cui: "[Relation between acute respiratory infection and enterovirus in children in Beijing area.]" in: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, Vol. 21, Issue 4, pp. 316-8, (2008) (PubMed).
抗原

ATG7 (ATG7 Autophagy Related 7 (ATG7))

别名

APG7L / ATG7 (ATG7 产品)

别名

APG7-LIKE antibody, APG7L antibody, GSA7 antibody, D8 antibody, UBA1B antibody, UBE1L antibody, UBE2 antibody, Ube1l antibody, 1300004C08Rik antibody, AO090001000744 antibody, atg7 antibody, 1810013K23Rik antibody, Agp7 antibody, Apg7l antibody, Atg7l antibody, ATAPG7 antibody, ATATG7 antibody, ATG7 antibody, AUTOPHAGY 7 antibody, AUTOPHAGY-RELATED 7 antibody, K15I22.10 antibody, K15I22_10 antibody, autophagy related 7 antibody, ubiquitin like modifier activating enzyme 7 antibody, ubiquitin-like modifier activating enzyme 7 antibody, autophagy-related protein 7 antibody, Autophagy-related protein 7 antibody, Apg7p antibody, ThiF family protein antibody, ubiquitin-like conjugating enzyme Atg7 antibody, ATG7 antibody, UBA7 antibody, Uba7 antibody, ANI_1_3368024 antibody, AOR_1_1338164 antibody, PTRG_02236 antibody, PAAG_00287 antibody, PITG_12537 antibody, atg7 antibody, Atg7 antibody, APG7 antibody

背景

Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein

基因ID

10533

NCBI登录号

NP_001129503

UniProt

O95352

途径

Response to Water Deprivation, Autophagy