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- 抗原 See all MIF 抗体
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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抗原表位
- AA 2-32
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适用
- 人
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宿主
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小鸡
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克隆类型
- 多克隆
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标记
- This MIF antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- P-M-F-I-V-N-T-N-V-P-R-A-S-V-P-D-G-F-L-S-E-L-T-Q-Q-L-A-Q-A-T-G
- 特异性
- This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically. Endotoxin Content: < 10 pg/μl by LAL method.
- 纯化方法
- Multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis
- 免疫原
- This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
- Top Product
- Discover our top product MIF Primary Antibody
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anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 3-111) antibody
MIF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) antibodyMIF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) antibodyMIF 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) antibodyMIF 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 2A10-4D3 unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) antibodyMIF 适用: 人 WB, ELISA, ICC, IHC (p), IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 51-115) antibodyMIF 适用: 人, 大鼠 WB, ELISA, IHC (p), FACS, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibodyVerified MIF 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibodyMIF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (C-Term) antibodyMIF 适用: 人, 大鼠 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) antibodyMIF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This IgY fraction antibody of anti-Human MacrophageMigration Inhibitory Factor (MIF) has been tested for use in ELISA and Immunoblotting. Although not tested, this antibody may also be useful for Neutralization assays,Immunohistochemistry and Flow Cytometry. The antibody recognizes 12,300 MW maturehuman MIF. The MIF gene encodesa protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown. Immunoblot: Using IRDYE800 conjugated Goat-a-Chicken IgG [H&L] MX10. A workingdilution range of 1: 500-1: 1,000 is suggested for this application to detect human MIF fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serumplus 10 ng/mL E. coli LPS. This product has been assayed by ELISA against human MIF using HRP ConjugatedGoat-a-Chicken IgG [H&L] MX10 and ABTS as a substrate for 30 minutes at roomtemperature. A working dilution range of 1: 1,000-1: 2,000 is suggested for this product. For use in ELISAformats, this antibody is best used as the second antibody in combination with amonoclonal antibody as a capture antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- 别名
- MIF (MIF 产品)
- 别名
- mif antibody, Mif antibody, gif antibody, glif antibody, mmif antibody, LOC100136498 antibody, LOC100284350 antibody, LOC100284546 antibody, GIF antibody, Glif antibody, GLIF antibody, MMIF antibody, macrophage migration inhibitory factor L homeolog antibody, macrophage migration inhibitory factor antibody, macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, mif.L antibody, mif antibody, MIF antibody, Mif antibody, PHATRDRAFT_49660 antibody, LOC100136498 antibody, cl405_1 antibody, LOC100284546 antibody
- 背景
- Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/mL to 10 ng/mL of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18- kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
- 基因ID
- 4282
- NCBI登录号
- NP_002406
- UniProt
- P14174
- 途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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