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MDM2 抗体 (pSer185)

Name: MDM2 抗体 (pSer185)
SKU: ABIN118010
Availability: OutOfStock

MDM2 适用: 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated

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产品编号 ABIN118010

发货至: 中国

Datasheet as PDF

抗原 See all MDM2 抗体

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
抗原表位
26

25

14

11

10

10

10

9

9

8

8

4

4

4

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 177-198, pSer185
适用
195

80

55

11

2

1

1

1

1

1
小鼠
宿主
170

28

1
兔
Compatible Secondaries
克隆类型
170

29
多克隆
标记
100

13

9

9

7

6

6

6

6

6

6

6

2

2

2

2

2

2

2

1

1

1

1

1
This MDM2 antibody is un-conjugated
应用范围
126

77

64

38

29

27

27

18

18

14

10

10

5

4

3

2

2

1

1

1

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

This affinity-purified antibody is directed against the phosphorylated form of mouse MDM2 protein at the pS185 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against Mouse MDM2 pS185 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot. A BLAST analysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.
Cellular Localization: Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleus. The nucleolar localization signals in both ARF and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

纯化方法

Immunoaffinity chromatography.

免疫原

Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 177-198 of mouse MDM2.

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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody
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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer166) antibody
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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 26-169) antibody
MDM2 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C1E3 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 393-424) antibody
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anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (pSer186) antibody
MDM2 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibody
MDM2 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) antibody
MDM2 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 119-438) antibody
MDM2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated

anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (AA 200-430) antibody
MDM2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

This affinity purified antibody has been tested for use in ELISA (1: 3,000 to 1: 12,000) andWestern blotting (1/500-1: 2,000). Western blotting shows specific banding forphosphorylated MDM 2 showing a band at approximately 102 kD corresponding tophosphorylated mouse MDM2. (See ref.5) for a discussion on expected molecular weights.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.33 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 2-8 °C.
抗原

MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

别名

MDM2 (MDM2 产品)

别名

ACTFS antibody, HDMX antibody, hdm2 antibody, zgc:109834 antibody, 1700007J15Rik antibody, AA415488 antibody, Mdm-2 antibody, MDM2 proto-oncogene antibody, MDM2 proto-oncogene S homeolog antibody, Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse) antibody, MDM2 oncogene, E3 ubiquitin protein ligase antibody, transformed mouse 3T3 cell double minute 2 antibody, MDM2 antibody, mdm2.S antibody, Mdm2 antibody, mdm2 antibody

背景

MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation. MDM2 binds to p53 and amplification of MDM2 in sarcomas leads to escape from p53-regulated growth control. This mechanism of tumorigenesis parallels that for virusinduced tumors in which viral oncogene products bind to and functionally inactivate p53. Overexpression of the MDM2 oncogene was found in leukemias. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. MDM2 interacts physically and functionally with the retinoblastoma (RB) protein and can inhibit its growth regulatory capacity. Both RB and p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2-p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. Four out of five of these alternatively spliced forms (MDM2a-MDMd) are missing substantial portions of the p53 binding domain and retain the acidic domain and the zinc-finger domains. The fifth and smallest transcript (MDM2e) retains the largest spliced region encoding the p53 binding domain, however, it lacks the nuclear localization signal, the acidic domain and zinc-finger domains. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full-length MDM2 transcript is expressed in normal tissue.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

基因ID

17246

NCBI登录号

NP_034916

UniProt

P23804

途径

p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway