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- 抗原 See all Merlin (NF2) 抗体
- Merlin (NF2) (Neurofibromin 2 (NF2))
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抗原表位
- pSer518
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Merlin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue.
- 纯化方法
- Affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
- 免疫原
- Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
- Top Product
- Discover our top product NF2 Primary Antibody
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anti-Neurofibromin 2 (NF2) (AA 477-576) antibody
NF2 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (AA 30-239) antibodyNF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (AA 30-311) antibodyNF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (AA 230-414) antibodyNF2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (pSer518) antibodyNF2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (AA 1-507) antibodyNF2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (pSer518) antibodyNF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (pSer518) antibodyNF2 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) antibodyNF2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Neurofibromin 2 (NF2) (C-Term) antibodyNF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. ByELISA, the antibody was found to be reactive with the phosphorylated form of theimmunizing peptide and minimally reactive with the nonphosphorylated form of theimmunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Althoughnot tested, this antibody is likely functional in immunohistochemistry andimmunoprecipitation. This product has been assayed against 0.1 g of phosphorylated peptide in a standardcapture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 4,000 is suggested for this product. Less than 5 % cross-reactivity was detected against the nonphosphorylated form of theimmunizing peptide. Dilute the antibody 1: 1,000 for immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.24 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
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- 抗原
- Merlin (NF2) (Neurofibromin 2 (NF2))
- 别名
- Merlin (NF2 产品)
- 别名
- ACN antibody, BANF antibody, SCH antibody, NF2 antibody, merlin antibody, zgc:194620 antibody, zgc:194631 antibody, NF2a antibody, nf2 antibody, neurofibromin 2 antibody, neurofibromin 2a (merlin) antibody, neurofibromin 2b (merlin) antibody, neurofibromin 2 (merlin) S homeolog antibody, NF2 antibody, Nf2 antibody, nf2a antibody, nf2b antibody, nf2.S antibody
- 背景
- The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM's suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa. This antibody is specific for the phosphorylated form of human NF2 (Merlin).Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin
- 基因ID
- 4771
- NCBI登录号
- NP_000259
- UniProt
- P35240
- 途径
- Cell-Cell Junction Organization
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