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- 抗原 See all SETDB1 抗体
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
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抗原表位
- AA 1058-1075
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SETDB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- Human ESET, 1291 aa, predicted MW 143.2 kDa: 1 msslpgcigl daatatvese eiaelqqavv eelgismeel rhfideelek mdcvqqrkkq 61 laeletwviq kesevahvdq fddasravt nceslvkdfy sklglqyrds ssedessrpt 121 eiieipdedd dvlsidsgda srtpkdqkl reamaalrks aqdvqkfmda vnkksssqdl 181 hkgtlsqmsg elskdgdliv smrilgkkrt ktwhkgtlia iqtvgpgkky kvkfdnkgks 241 llsgnhiayd yhppadklyv gsrvvakykd gnqvwlyagi vaetpnvknk lrfliffddg 301 yasyvtqsel ypicrplkkt wediediscr dfieeyvtay pnrpmvllks gqlikteweg 361 twwksrveev dgslvrilfl ddkrcewiyr gstrlepmfs mktssasale kkqgqlrtrp 421 mgavrskgp vvqytqdltg tgtqfkpvep pqptappapp fppapplspq agdsdlesql 481 qsrkqvakk stsfrpgsvg sghssptspa lsenvsggkp ginqtyrspl gstasapaps 541 alpappappv fhgmlerapa epsyrapmek lfylphvcsy tclsrvrpmr neqyrgknpl 601 lvpllydfrr mtarrrvnrk mgfhviyktp cglclrtmqe ierylfetgc dflflemfcl 661 dpyvlvdrkf qpykpfyyil ditygkedvp lscvneidtt pppqvayske ripgkgvfin 721 tgpeflvgcd ckdgcrdksk cachqltiqa tactpggqin pnsgyqykrl eeclptgvye 781 cnkrckcdpn mctnrlvqhg lqvrlqlfkt qnkgwgircl ddiakgsfvc iyagkiltdd 841 fadkeglemg deyfanldhi esvenfkegy esdapcssds sgvdlkdqed gnsgtedpee 901 snddssddnf ckdedfstss vwrsyatrrq trgqkengls ettskdshpp dlgpphipvp 961 psipvggcnp psseetpknk vaswlscnsv seggfadsds hssfktnegg egraggsrme 1021 aekastsglg ikdegdikqa kkedtddrnk msvvtessrn ygynpspvkp eglrrppskt 1081 smhqsrrlma saqsnpddvl tlsssteseg esgtsrkpta gqtsatavds ddiqtissgs 1141 egddfedkkn mtgpmkrqva vkstrgfalk sthgiaikst nmasvdkges apvrkntrqf 1201 dgeescyii daklegnlgr ylnhscspnl fvqnvfvdth dlrfpwvaff askriragte 1261 ltwdynyevg svegkellcc cgaiecrgrl l
- 特异性
- This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein. Cross reactivity with ESET protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1058-1075 of human ESET.
- Top Product
- Discover our top product SETDB1 Primary Antibody
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anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-100) antibody
SETDB1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4A3 unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1-397) antibodySETDB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) antibodySETDB1 适用: 人, 小鼠, 猴 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 5H6A12 unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) antibodySETDB1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 290-437) antibodySETDB1 适用: 人 IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) antibodySETDB1 适用: 人, 小鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 5H6D4 unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1058-1075) antibodySETDB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 1193-1225) antibodySETDB1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SET Domain, Bifurcated 1 (SETDB1) (AA 2-100) antibodySETDB1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) andWestern blotting (1: 1,000-1: 2,500). Western blotting shows reactivity specific for human ESET detecting a band atapproximately 170 kDa. Specific band detection by Western blot is blocked by peptidecompetition by pre-incubating the antibody with the immunizing peptide prior to reactionwith the membrane. Reactivity in other immunoassays is unknown.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.17 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
- 别名
- SETDB1 (SETDB1 产品)
- 别名
- ESET antibody, H3-K9-HMTase4 antibody, KG1T antibody, KMT1E antibody, TDRD21 antibody, AU022152 antibody, mKIAA0067 antibody, SETDB1 antibody, NV10069 antibody, fc07d03 antibody, si:ch211-81a5.6 antibody, wu:fc07d03 antibody, im:7143091 antibody, wu:fa04a12 antibody, wu:fj80h02 antibody, zgc:152899 antibody, SET domain bifurcated 1 antibody, SET domain, bifurcated 1 antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 antibody, histone-lysine N-methyltransferase SETDB1 antibody, SET domain, bifurcated 1a antibody, SET domain bifurcated 1 L homeolog antibody, SET domain, bifurcated 1b antibody, SETDB1 antibody, Setdb1 antibody, MLLT11 antibody, LOC100120014 antibody, LOC100461671 antibody, setdb1a antibody, setdb1.L antibody, setdb1b antibody
- 背景
- The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.Synonyms: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, Histone-lysine N-methyltransferase SETDB1, KIAA0067, KMT1E, Lysine N-methyltransferase 1E, SET domain bifurcated 1
- 基因ID
- 9869
- NCBI登录号
- NP_001138887
- UniProt
- Q15047
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