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GFP 抗体 (FITC)

Name: GFP 抗体 (FITC)
SKU: ABIN117893
Availability: OutOfStock

GFP 适用: Aequorea victoria IF 宿主: 小鼠 Monoclonal 9F9-F9 FITC

产品编号 ABIN117893

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
175

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
宿主
74

73

35

11

9

6

2

1
小鼠
Compatible Secondaries
克隆类型
121

85
单克隆
标记
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to FITC
应用范围
173

87

55

42

33

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Immunofluorescence (IF)

特异性

This product was prepared from tissue culture supernatant by Protein A chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against wild type, recombinant and enhanced forms of GFP.

产品特性

Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 3.5 moles FITC per mole of Mouse IgG).

纯化方法

Protein A chromatography.

免疫原

The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

克隆位点

9F9-F9

亚型

IgG2a

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应用备注

Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISAfor direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP.

限制

仅限研究用
溶解方式

Restore with 1.0 mL of deionized water (or equivalent).

浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Dilute only prior to immediate use

储存条件

4 °C/-20 °C

储存方法

Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

物质类

Viral Protein

背景

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

基因ID

6100

UniProt

P42212