(3 references)-
- 抗原 See all IL-2 (IL2) 抗体
- IL-2 (IL2) (Interleukin 2 (IL2))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-2. Neutralization activity may be measured with a proliferation assay using 80 pg/mL recombinant mouse IL-2 (Cat. No. 550069) to stimulate CTLL-2 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-2 for 60 minutes).
50% Neutralization (ND50) at 5 - 30 ng/mL
>95% Neutralization at 30 - 80 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- T cell clone supernatant
- 克隆位点
- S4B6
- 亚型
- IgG2a
- Top Product
- Discover our top product IL2 Primary Antibody
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anti-Interleukin 2 (IL2) (AA 21-153) antibody
IL2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
anti-Interleukin 2 (IL2) (AA 10-169) antibodyIL2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS, IP, ICC 宿主: 小鼠 Monoclonal 35C3 unconjugated
anti-Interleukin 2 (IL2) antibody (APC)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 APC
anti-Interleukin 2 (IL2) antibody (PE)IL2 适用: 人 FACS 宿主: 小鼠 Monoclonal 35C3 PE
anti-Interleukin 2 (IL2) (AA 21-153) antibodyIL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 (IL2) antibodyIL2 适用: 人 WB, ELISA, FACS 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Western Blot: The S4B6 antibody (purified format) has been reported to be useful for Western blotting. A concentration of 1-5 µg/mL (in conjunction with an AKP-conjugated goat anti-rat Ig secondary antibody) has been reported to enable detection of ≤ 100 ng/lane of recombinant mouse IL-2 (Cat. No. 550069) under reducing conditions.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells." in: International immunology, Vol. 5, Issue 8, pp. 877-83, (1993) (PubMed).
: "Polymorphism of age-related changes in interleukin (IL) production: differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4." in: European journal of immunology, Vol. 20, Issue 6, pp. 1289-96, (1990) (PubMed).
: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).
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: "Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells." in: International immunology, Vol. 5, Issue 8, pp. 877-83, (1993) (PubMed).
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- 抗原
- IL-2 (IL2) (Interleukin 2 (IL2))
- 别名
- IL-2 (IL2 产品)
- 别名
- IL2 antibody, IL-2 antibody, il2 antibody, TCGF antibody, lymphokine antibody, Il-2 antibody, POIL2 antibody, interleukin 2 antibody, interleukin-15 antibody, IL2 antibody, il2 antibody, LOC100303688 antibody, Il2 antibody
- 背景
- The S4B6 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the S4B6 hyridoma was a T cell clone supernatant. This is a neutralizing antibody.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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