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IRF7 抗体 (pSer477) (Alexa Fluor 488)

IRF7 适用: 人 ICS 宿主: 小鼠 Monoclonal K47-671 Alexa Fluor 488
产品编号 ABIN1177072
发货至: 中国
  • 抗原 See all IRF7 抗体
    IRF7 (Interferon Regulatory Factor 7 (IRF7))
    抗原表位
    • 17
    • 17
    • 15
    • 8
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer477
    适用
    • 70
    • 55
    • 54
    • 17
    • 8
    • 3
    • 2
    • 2
    • 1
    宿主
    • 69
    • 2
    小鼠
    克隆类型
    • 58
    • 13
    单克隆
    标记
    • 24
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
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    • 1
    This IRF7 antibody is conjugated to Alexa Fluor 488
    应用范围
    • 50
    • 18
    • 15
    • 14
    • 14
    • 13
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    Intracellular Staining (ICS)
    品牌
    BD Phosflow™
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Phosphorylated Human IRF-7 Peptide
    克隆位点
    K47-671
    亚型
    IgG1 kappa
    Top Product
    Discover our top product IRF7 Primary Antibody
  • 样本量
    20 μL
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
  • Paz, Sun, Nakhaei, Romieu-Mourez, Goubau, Julkunen, Lin, Hiscott: "Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway." in: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 52, Issue 1, pp. 17-28, (2006) (PubMed).

    Hoshino, Sugiyama, Matsumoto, Tanaka, Saito, Hemmi, Ohara, Akira, Kaisho: "IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9." in: Nature, Vol. 440, Issue 7086, pp. 949-53, (2006) (PubMed).

    Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, (2003) (PubMed).

  • 抗原
    IRF7 (Interferon Regulatory Factor 7 (IRF7))
    别名
    IRF-7 (IRF7 产品)
    别名
    wu:fl24c03 antibody, zgc:66043 antibody, zgc:77947 antibody, IRF7 antibody, irf7 antibody, IRF-7H antibody, IRF7A antibody, IRF7B antibody, IRF7C antibody, IRF7H antibody, interferon regulatory factor 7 antibody, irf7 antibody, IRF7 antibody, Irf7 antibody
    背景
    Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K47-671 monoclonal antibody recognizes human IRF-7 phosphorylated at serines 477 and 479 (pS477/pS479). Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K47-671 does not cross-react with TBK1 by Western blot analysis using the purified antibody.
    途径
    TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagy
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