-
- 抗原 See all IL-4 (IL4) 抗体
- IL-4 (IL4) (Interleukin 4 (IL4))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This IL-4 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
-
Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-4 for 60 minutes).
50% Neutralization (ND50) at 1 - 50 ng/mL
>95% Neutralization at 50 - 300 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Partially Purified Mouse IL-4
- 克隆位点
- 11B11
- 亚型
- IgG1
- Top Product
- Discover our top product IL4 Primary Antibody
-
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
-
-
Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, (1995) (PubMed).
: "Enhanced IL-4-mediated D10.G4.1 proliferation with suboptimal concentrations of anti-IL-4 receptor monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 394-8, (1993) (PubMed).
: "Administration of anti-IL-4 monoclonal antibody 11B11 increases the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 3978-85, (1992) (PubMed).
: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, (1991) (PubMed).
: "Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1." in: Nature, Vol. 315, Issue 6017, pp. 333-6, (1985) (PubMed).
: "
-
Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, (1995) (PubMed).
-
- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL-4 (IL4 产品)
- 别名
- IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
- 背景
- The 11B11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 from PMA-stimulated EL-4 supernatant. The purified or unconjugated form of this antibody has been reported to be neutralizing.
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
-