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- 抗原 See all IL-9 (IL9) 抗体
- IL-9 (IL9) (Interleukin 9 (IL9))
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适用
- 小鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This IL-9 antibody is un-conjugated
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应用范围
- ELISA, Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-9. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant mouse IL-9 to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-9 for 60 minutes).
50% Neutralization (ND50) at 5 - 25 ng/mL
>95% Neutralization at 50 - 250 ng/mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Mouse IL-9 Recombinant Protein
- 克隆位点
- D9302C12
- 亚型
- IgG2 kappa
- Top Product
- Discover our top product IL9 Primary Antibody
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- 应用备注
- ELISA: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the purified D8402E8 antibody (Cat. No. 551218) as the capture antibody, with recombinant mouse IL-9 as the standard.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis." in: Journal of leukocyte biology, Vol. 57, Issue 3, pp. 353-60, (1995) (PubMed).
: "Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells." in: Nature, Vol. 291, Issue 5813, pp. 332-4, (1981) (PubMed).
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Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- 抗原
- IL-9 (IL9) (Interleukin 9 (IL9))
- 别名
- IL-9 (IL9 产品)
- 别名
- IL9 antibody, IL-9 antibody, Il-9 antibody, P40 antibody, HP40 antibody, interleukin 9 antibody, IL9 antibody, il9 antibody, Il9 antibody
- 背景
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The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Ralpha) and common gamma chain (gammac) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.
Synonyms: IL-9, Interleukin-9, MEA, P40, T-cell growth factor P40, TCGF III - 途径
- JAK/STAT Signaling
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