山羊 anti-人 IgA, IgG, IgM (Whole Molecule) Antibody (Biotin)

ABIN117310 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Whole Molecule
106
78
52
52
30
11
适用

229
172
79
60
29
26
10
8
7
7
6
5
3
3
3
宿主
山羊
506
107
12
8
4
2
2
克隆类型
多克隆
标记
Biotin
103
103
80
48
33
32
18
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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免疫原 Human IgG, IgA and IgM whole molecules
产品特性 Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
纯化方法 Immunoaffinity chromatography
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
背景 Synonyms: Human IgA, Human IgG, Human IgM
应用备注 Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at roomtemperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL deionized water or equivalent.
浓度 2.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
背景 Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).