山羊 anti-雪貂 IgG (Chain gamma) Antibody (HRP)

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抗原
抗原表位
Chain gamma
5028
2578
1931
1069
1007
857
329
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用
雪貂
2912
2859
2405
1752
1485
796
623
442
381
380
355
340
307
293
208
148
139
114
41
28
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主
山羊
6892
4912
1878
895
703
401
92
54
52
21
9
8
5
5
2
1
1
克隆类型
多克隆
标记
HRP
2234
1889
1740
1374
765
679
519
300
299
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
应用范围
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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免疫原 Ferret IgG gamma (gamma chain)
纯化方法 Prepared from monospecific antiserum by Immunoaffinity Chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
背景 Synonyms: Ferret Immunoglobulin G
研究领域 Immunology, Secondary Antibodies
应用备注 Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL of deionized water or equivalent.
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
储存液 Thimerosal (Merthiolate)
注意事项 Biohazard Informations: This product contains thimerosal which is hazardous.
注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件 4 °C/-20 °C
储存方法 Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -28 °C or below.
背景 Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).