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山羊 anti-雪貂 IgG (Chain gamma) Antibody (HRP)

ABIN117102 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Chain gamma
4684
2573
1882
1039
1015
825
304
273
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用
雪貂
2780
2760
2347
1724
1436
778
607
426
364
338
330
324
307
290
169
148
126
114
41
28
27
25
12
11
9
8
6
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
宿主
山羊
6749
4661
1834
812
684
394
88
52
51
9
8
5
5
4
2
1
1
克隆类型
多克隆
标记
HRP
2182
1862
1718
1362
765
675
521
297
295
284
274
268
217
186
178
117
102
79
69
58
54
40
35
26
23
22
19
19
19
19
19
16
15
15
15
15
15
15
14
13
12
11
11
10
10
9
9
9
9
9
9
9
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
应用范围
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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免疫原 Ferret IgG gamma (gamma chain)
纯化方法 Prepared from monospecific antiserum by Immunoaffinity Chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
背景 Synonyms: Ferret Immunoglobulin G
研究领域 Immunology, Secondary Antibodies
应用备注 Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL of deionized water or equivalent.
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
储存液 Thimerosal (Merthiolate)
注意事项 Biohazard Informations: This product contains thimerosal which is hazardous.
注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件 4 °C/-20 °C
储存方法 Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -28 °C or below.
背景 Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).