电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Avidin 抗体 (Biotin)

AVD 适用: 小鸡 WB, IHC (fro), IHC (p), EIA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN116961
发货至: 中国
  • 抗原 See all Avidin (AVD) 抗体
    Avidin (AVD)
    适用
    • 8
    • 7
    • 4
    小鸡
    宿主
    • 10
    • 7
    • 1
    克隆类型
    • 11
    • 7
    多克隆
    标记
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This Avidin antibody is conjugated to Biotin
    应用范围
    • 13
    • 13
    • 10
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
    产品特性
    Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
    纯化方法
    A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
    免疫原
    Avidin [Hen Egg White]
    Top Product
    Discover our top product AVD Primary Antibody
  • 应用备注
    Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Avidin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 32,000 of the reconstitution concentration is suggestedfor this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 1.0 mL of deionized water (or equivalent).
    浓度
    10.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Do NOT add sodium azide! Dilute only prior to immediate use.
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
  • 抗原
    Avidin (AVD)
    别名
    Avidin (AVD 产品)
    别名
    avidin antibody, avd antibody, LOC723980 antibody, AVD antibody
    背景
    The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
    基因ID
    396260
    NCBI登录号
    NP_990651
    UniProt
    P02701
You are here:
客服