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Avidin 抗体 (Biotin)

Details for Product anti-Avidin Antibody No. ABIN116961, 供应商: Log in to see
抗原
  • avidin
  • avd
  • LOC723980
  • AVD
适用
小鸡
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26
10
9
8
宿主

43
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2
克隆类型
多克隆
标记
This Avidin antibody is conjugated to Biotin
12
9
9
4
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4
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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58
10
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8
5
4
4
3
2
2
2
1
1
Supplier
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免疫原 Avidin [Hen Egg White]
特异性 Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
产品特性 Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
纯化方法 A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
背景 The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
基因ID 396260
NCBI登录号 NP_990651
UniProt P02701
研究领域 Epitope Tags, Tags/Labels
应用备注 Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Avidin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 32,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL of deionized water (or equivalent).
浓度 10.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. Do NOT add sodium azide! Dilute only prior to immediate use.
储存条件 4 °C/-20 °C
储存方法 Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
背景 Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).