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- 抗原 See all ATG12 抗体
- ATG12 (Autophagy Related 12 (ATG12))
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适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATG12 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 交叉反应 （详细）
- Species reactivity (tested):S. cerevisiae, Yeast
- 纯化方法
- A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.
- 免疫原
- This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.
- Top Product
- Discover our top product ATG12 Primary Antibody
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anti-Autophagy Related 12 (ATG12) (N-Term) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, 小鸡, 兔, Pig, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 43CT73-3-5-5-4 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 101-187) antibodyATG12 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-30), (N-Term) antibodyATG12 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7475 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-50) antibodyATG12 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 43CT73-3-5-5-4 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-140) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 79-128) antibodyATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 猴, 小鸡, 兔, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water or equivalent.
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- ATG12 (Autophagy Related 12 (ATG12))
- 别名
- ATG12 / APG12 (ATG12 产品)
- 别名
- APG12 antibody, APG12L antibody, FBR93 antibody, HAPG12 antibody, Apg12l antibody, AUTOPHAGY 12 antibody, AUTOPHAGY 12 B antibody, CG10861 antibody, Dmel\\CG10861 antibody, atg12 antibody, ATG12 antibody, apg12 antibody, DDBDRAFT_0185323 antibody, DDBDRAFT_0191412 antibody, DDB_0185323 antibody, DDB_0191412 antibody, 4931423H11Rik antibody, A330058M13Rik antibody, Atg12l antibody, AO090001000593 antibody, autophagy related 12 antibody, Ubiquitin-like superfamily protein antibody, Atg12p antibody, autophagy protein antibody, ubiquitin-like protein ATG12B antibody, autophagy associated protein Atg12 antibody, Autophagy-related 12 antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) antibody, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene antibody, autophagy associated ubiquitin-like modifier Atg12 antibody, hypothetical protein antibody, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) antibody, autophagy protein 12 antibody, autophagy related 12 S homeolog antibody, autophagy-related protein 12 antibody, ATG12 antibody, Atg12 antibody, APG12B antibody, APG12 antibody, LOC9321066 antibody, apg12 antibody, atg12 antibody, LOC743425 antibody, CAALFM_C113950CA antibody, atg12.S antibody, ANI_1_922094 antibody, AOR_1_1064164 antibody
- 背景
- Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12
- 基因ID
- 852518
- NCBI登录号
- NP_009776
- UniProt
- P38316
- 途径
- Autophagy
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