Recombinant Tubulin-GTP 抗体
适用: Drosophila melanogaster, 人, 小鼠, 大鼠
ICC
宿主: Human
Monoclonal
MB11
unconjugated
Recombinant Antibody
(3 references)-
- 抗原
- Tubulin-GTP
- 抗体类型
- Recombinant Antibody
- 适用
- Drosophila melanogaster, 人, 小鼠, 大鼠
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宿主
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Human
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunocytochemistry (ICC)
- 特异性
- Recognizes human, mouse, rat and drosophila tubulin-GTP.
- 交叉反应
- Fruit Fly (Drosophila melanogaster), 人, 小鼠, 大鼠
- 纯化方法
- Purified from HEK 293 cell culture supernatant.
- 纯度
- >95 % (SDS-PAGE)
- 免疫原
- Full length GTP-lambda-S loaded tubulin from pig brain.
- 克隆位点
- MB11
- 亚型
- IgG2
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-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. - 有效期
- 12 months
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: "Molecular understanding of label-free second harmonic imaging of microtubules." in: Nature communications, Vol. 10, Issue 1, pp. 3530, (2019) (PubMed).
: "Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 245-55, (2011) (PubMed).
: "Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues." in: Science (New York, N.Y.), Vol. 322, Issue 5906, pp. 1353-6, (2008) (PubMed).
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: "Molecular understanding of label-free second harmonic imaging of microtubules." in: Nature communications, Vol. 10, Issue 1, pp. 3530, (2019) (PubMed).
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- 抗原
- Tubulin-GTP
- 背景
- Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
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