IKKi/IKKe 抗体 (pThr501)
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- 抗原 See all IKKi/IKKe (IKBKE) 抗体
- IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
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抗原表位
- pThr501
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IKKi/IKKe antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- R-S-R-L-R-pT-L-A-E-V-L
- 特异性
- This phospho specific polyclonal antibody is specific for phosphorylated pThr501 IKKE. Reactivity with non-phosphorylated IKKE is minimal.
- 纯化方法
- Immunoaffinity Chromatography against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.
- 免疫原
- IKKE phosphopeptide corresponding to a region of the human protein surrounding pThr501 conjugated to KLH. Genename: IKKe
- Top Product
- Discover our top product IKBKE Primary Antibody
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anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) antibody
IKBKE 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 495-716) antibodyIKBKE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (C-Term) antibodyIKBKE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 701-716) antibodyIKBKE 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 541-640) antibodyIKBKE 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F7 unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 544-680) antibodyIKBKE 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) antibodyIKBKE 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pThr501) antibodyIKBKE 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 1-257) antibodyIKBKE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B4B5 unconjugated
anti-Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (AA 686-716), (C-Term) antibodyIKBKE 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3912 unconjugated
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- 应用备注
- This phospho specific polyclonal antibody reacts with phosphorylated human IKKE atpT501 and shows minimal reactivity by Western blot with non-phosphorylated IKKE andminimal reactivity by ELISA against the non-phosphorylated form of the immunizingpeptide. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation.
- 限制
- 仅限研究用
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- 浓度
- 1.15 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
- 别名
- IKBKE / IKKE (IKBKE 产品)
- 别名
- zgc:100844 antibody, IKBKE antibody, MGC130907 antibody, IKK-E antibody, IKK-i antibody, IKKE antibody, IKKI antibody, AW558201 antibody, IKKepsilon antibody, Ikki antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antibody, inhibitor of kappaB kinase epsilon antibody, IKBKE antibody, ikbke antibody, ikbke.L antibody, Ikbke antibody
- 背景
- Nuclear Factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cytoplasm of the cell, which inhibit NF-κB activity. IκB proteins are phosphorylated by an IκB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-κB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IκBa.Synonyms: I kappa-B kinase epsilon, IKK-E, IKK-I, IKK-epsilon, IKKI, Inducible I kappa-B kinase, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151
- 基因ID
- 9641
- NCBI登录号
- NP_001180250
- UniProt
- Q14164
- 途径
- TLR signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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