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Golgin B1 (GOLGB1) 抗体 (Atto 488)

ABIN1169060 产品详细信息, 供应商: Log in to see
抗原
  • gcp
  • golim1
  • giantin
  • 4930428L02Rik
  • 628101
  • 6330407A06Rik
  • AU042952
  • C130074L01Rik
  • F730017E11Rik
  • Gm6840
  • mKIAA4151
  • GCP
  • GCP372
  • GOLIM1
  • golgin B1
  • golgin subfamily B member 1
  • golgi autoantigen, golgin subfamily b, macrogolgin 1
  • GOLGB1
  • golgb1
  • LOC100340420
  • Golgb1
适用
人, 小鼠
33
13
10
2
2
1
1
1
1
1
1
宿主
Human
17
13
2
2
2
1
抗体类型
Recombinant Antibody
克隆类型 (克隆位点)
单克隆 ()
标记
Atto 488
2
1
1
1
1
1
应用范围
Immunocytochemistry (ICC)
22
10
8
8
7
5
3
3
2
2
2
1
1
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免疫原 Human recombinant giantin.
克隆位点 TA10
亚型 IgG2
特异性 Recognizes human and mouse giantin.
交叉反应 人, 小鼠
纯化方法 Purified from HEK 293 cell culture supernatant.
纯度 >95 % (SDS-PAGE)
别名 Giantin (GOLGB1 Antibody 摘要)
背景 Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
UniProt Q14789
应用备注 Optimal working dilution should be determined by the investigator.
说明

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

限制 仅限研究用
浓度 1 mg/mL
缓冲液 In PBS containing 0.02 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.
有效期 12 months