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Laminin 抗体 (Biotin)

LN 适用: 人 WB, IHC (p), IP, IHC (fro), EIA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN116904
发货至: 中国
  • 抗原 See all Laminin (LN) 抗体
    Laminin (LN)
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Laminin antibody is conjugated to Biotin
    应用范围
    • 21
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    产品特性
    Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
    纯化方法
    Immunoaffinity chromatography.
    免疫原
    Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.
    Top Product
    Discover our top product LN Primary Antibody
  • 应用备注
    Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store the antibody (in aliquots) at -20 °C.
  • 抗原
    Laminin (LN)
    别名
    Laminin (LN 产品)
    别名
    LAMC1 antibody, laminin antibody, laminin, beta 2 (laminin S) antibody, LAMB2 antibody
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