GFP 抗体 (Texas Red (TR))
(12 references)-
- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GFP antibody is conjugated to Texas Red (TR)
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
- 产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.2 moles Texas Red per mole of Goat IgG. - 纯化方法
- Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Top Product
- Discover our top product GFP Primary Antibody
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP WB, ELISA, IF, IP, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibodyGFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)GFP 适用: Aequorea victoria FACS, IM 宿主: 山羊 Polyclonal FITC
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- 应用备注
- Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigenrecognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 witn 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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: "Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 2, pp. 418-32, (2015) (PubMed).
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: "Non-invasive monitoring of immunization progress in mice via IgG from feces." in: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).
: "Expression of luteinizing hormone receptors in the mouse penis." in: Journal of andrology, Vol. 32, Issue 1, pp. 49-54, (2011) (PubMed).
: "Tom20 mediates localization of mRNAs to mitochondria in a translation-dependent manner." in: Molecular and cellular biology, Vol. 30, Issue 1, pp. 284-94, (2010) (PubMed).
: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).
: "Molecular imaging of interstitial alterations in remodeling myocardium after myocardial infarction. ..." in: Journal of the American College of Cardiology, Vol. 52, Issue 24, pp. 2017-28, (2009) (PubMed).
: "Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response." in: Immunology, Vol. 121, Issue 2, pp. 174-82, (2007) (PubMed).
: "Butyrophilin controls milk fat globule secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 27, pp. 10385-10390, (2006) (PubMed).
: "Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection." in: Infection and immunity, Vol. 74, Issue 11, pp. 6505-8, (2006) (PubMed).
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: "Metabolic Suppression of a Drug-Resistant Subpopulation in Cancer Spheroid Cells." in: Journal of cellular biochemistry, Vol. 117, Issue 1, pp. 59-65, (2016) (PubMed).
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 别名
- green fluorescent protein antibody, gfp antibody
- 物质类
- Viral Protein
- 背景
- Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
- 基因ID
- 6100
- UniProt
- P42212
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