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GFP 抗体 (FITC)

GFP 适用: Aequorea victoria WB, IF, IHC (fro), EIA 宿主: 山羊 Polyclonal FITC
产品编号 ABIN116747
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
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    Aequorea victoria
    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This GFP antibody is conjugated to FITC
    应用范围
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
    产品特性
    Absorption / Emission: 495 nm / 528 nm
    Molar Ratio: 4.6 moles FITC per mole of Goat IgG
    纯化方法
    Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
    免疫原
    GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody canbe used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen andrecognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a sandwich ELISA is well suited to
    限制
    仅限研究用
  • 溶解方式
    Restore with 1.0 mL of deionized water or equivalent.
    浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and Protease free) and 0.01 % (w/v) Sodium Azide as preservative
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Dilute only prior to immediate use.
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below.
  • Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, (2012) (PubMed).

    Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

    Kofidis, de Bruin, Yamane, Tanaka, Lebl, Swijnenburg, Weissman, Robbins: "Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury." in: Circulation, Vol. 111, Issue 19, pp. 2486-93, (2005) (PubMed).

  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    别名
    GFP (GFP 产品)
    别名
    green fluorescent protein antibody, gfp antibody
    物质类
    Viral Protein
    背景
    Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
    基因ID
    6100
    UniProt
    P42212
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