GFP 抗体 (Green Fluorescent Protein) (Biotin)

Details for Product anti-GFP Antibody No. ABIN116746, 供应商: Log in to see
抗原
  • green fluorescent protein
  • gfp
适用
Aequorea victoria
641
23
16
5
5
2
2
1
1
宿主
山羊
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171
82
41
16
9
9
2
克隆类型
多克隆
标记
This GFP antibody is conjugated to Biotin
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31
28
14
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1
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应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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2
2
1
1
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选项
Supplier
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免疫原 GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
特异性 This GFP antibody was prepared from monospecific antiserum by Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
交叉反应 (详细) Ads. to Hu, Ms, Rt Serum Proteins
产品特性 Molar Ratio: 10-20 BAC molecules per Goat IgG molecule
纯化方法 Immunoaffinity Chromatography
别名 GFP
物质类 Tag
背景 Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
基因ID 6100
UniProt P42212
研究领域 Tags/Labels
应用备注 Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody canbe used to detect GFP by ELISA (Sandwich or Capture) for the direct binding of antigen andrecognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well suited totitrate GFP in solution using this antibody in combination with a monoclonal anti-GFPantibody(Cat#R1461P) using either form of the antibody as the capture or detection
限制 仅限研究用
溶解方式 Restore with 1.0 mL of deionized water (or equivalent).
浓度 1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabilizer.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件 -20 °C
储存方法 Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
有引用在: Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, 2012 (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, 2009 (PubMed).

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