Collagen Type I 抗体 (Biotin)
(4 references)-
- 抗原 See all Collagen Type I (COL1) 抗体
- Collagen Type I (COL1) (Collagen, Type I (COL1))
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适用
- 人, 大鼠, 小鼠, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Collagen Type I antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 特异性
- This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
- 纯化方法
- Immunoaffinity Chromatography.
- 免疫原
- Collagen Type I from human and bovine placenta.
- Top Product
- Discover our top product COL1 Primary Antibody
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anti-Collagen, Type I (COL1) antibody
COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1321-1400) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔, 犬, 鱼 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) (AA 1051-1150) antibodyCOL1 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc), ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type I (COL1) antibody (FITC)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, IHC, IP, FACS 宿主: 兔 Polyclonal FITC
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP, FACS 宿主: 兔 Polyclonal Biotin
anti-Collagen, Type I (COL1) antibody (HRP)COL1 适用: 人, 大鼠, 小鼠, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal HRP
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Collagen, Type I (COL1) antibodyCOL1 适用: 人, 大鼠, 小鼠 IHC (p), IF 宿主: 小鼠 Monoclonal 4H10 unconjugated
anti-Collagen, Type I (COL1) antibody (Biotin)COL1 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
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- 应用备注
- Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/3,000-1/6,000. Western blot: 1/3,000-1/6,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use. . Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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: "The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15821-44, (2015) (PubMed).
: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).
: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).
: "Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24506-13, (2008) (PubMed).
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: "The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15821-44, (2015) (PubMed).
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- 抗原
- Collagen Type I (COL1) (Collagen, Type I (COL1))
- 别名
- Collagen Type I (COL1 产品)
- 别名
- collagen type III alpha 1 chain antibody, COL3A1 antibody
- 背景
- Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
- 基因ID
- 1277
- NCBI登录号
- NP_000079
- UniProt
- P02452
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