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CXCL1 抗体

CXCL1 适用: 人 WB, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116259
发货至: 中国
  • 抗原 See all CXCL1 抗体
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This CXCL1 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    特异性
    This antibody is specific for Human Growth Related Oncoprotein alpha. Other species not tested.
    纯化方法
    Affinity Chromatography immobilized Human GRO/MGSA matrix
    免疫原
    Highly pure (>98%) E.coli derived recombinant Human GRO/MGSA (Cat.-No PA062). Remarks: Molecular Weight: ~150 kDa
    Top Product
    Discover our top product CXCL1 Primary Antibody
  • 应用备注
    NeutralisationTo yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5-3.0 μg/mL of this antibody is required. Sandwich ELISATo detect Human GRO/MGSA by Sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated anti Human GRO/MGSA (PP1080B1 orPP1080B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hGRO/MGSA. Western blotTo detect Human GRO/MGSA by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGRO/MGSA is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. Immunohistochemistry on Frozen and Paraffin Embedded Sections. A starting dilution of0.5-2 μg is recommended.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • 抗原
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    别名
    CXCL1 / GRO-alpha (CXCL1 产品)
    别名
    FSP antibody, GRO1 antibody, GROa antibody, MGSA antibody, MGSA-a antibody, NAP-3 antibody, SCYB1 antibody, CINC-1 antibody, Gro1 antibody, CXCL1 antibody, Sdf1 antibody, Fsp antibody, KC antibody, Mgsa antibody, N51 antibody, Scyb1 antibody, gro antibody, GRO antibody, Gro antibody, C-X-C motif chemokine ligand 1 antibody, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antibody, C-X-C motif chemokine ligand 12 antibody, chemokine (C-X-C motif) ligand 1 antibody, CXCL1 antibody, Cxcl1 antibody, Cxcl12 antibody, GRO1 antibody
    背景
    Three Human MGSA/GRO genes encode 3 highly related chemokines, MGSA/GRO alpha, -beta and -gamma. All 3 MGSA/GRO proteins bind to the same receptors, but with differing affinities, and stimulate a number of biological responses including chemotaxis, angiogenesis, and growth regulation. Melanoma growth stimulatory activity/gro alpha (MGSA), a member of the alpha-chemokine family, is produced by a variety of dermal and epidermal cells and can act in a paracrine and autocrine fashion.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1
    基因ID
    2919
    NCBI登录号
    NP_001502
    UniProt
    P09341
    途径
    Autophagy
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