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Chemokine (C-C Motif) Ligand 8 (CCL8) 抗体

ABIN116143 产品详细信息, 供应商: Log in to see
抗原
  • HC14
  • MCP-2
  • MCP2
  • SCYA10
  • SCYA8
  • 1810063B20Rik
  • AB023418
  • Mcp2
  • Scya8
  • chemokine (C-C motif) ligand 8
  • monocyte chemoattractant protein-2 precursor
  • CCL8
  • Ccl8
  • MCP-2
适用

91
51
3
2
1
宿主

72
39
15
1
克隆类型
多克隆
标记
非结合性
15
7
6
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
101
57
15
14
8
6
4
4
4
2
2
1
1
Supplier
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免疫原 Highly pure (>98%) recombinant hMCP-2.
特异性 Human Macrophage Chemotactic Protein-2 (MCP-2).
纯化方法 Affinity chromatography.
别名 MCP2 / CCL8 (CCL8 Antibody 摘要)
背景 MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8
基因ID 6355
NCBI登录号 NP_005614
UniProt P80075
研究领域 Cytokines, Chemokines
应用备注 Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMCP-2 (100.00 ng/mL), a concentration of 12-16 μg/mL of this antibody is required. ELISA: To detect hMCP-2 by Indirect ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5-0.2 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMCP-2. Sandwich: To detect hMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human MCP-2 (PP1045B1/B2) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthMCP-2. Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液 PBS, pH 7.2 without preservatives.
储存液 Without preservative
注意事项 Centrifuge vial prior to opening!
储存条件 4 °C
储存方法 Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.