EGF 抗体 (Biotin)
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- 抗原 See all EGF 抗体
- EGF (Epidermal Growth Factor (EGF))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EGF antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts with Epidermal Growth Factor (hEGF).
- 纯化方法
- Affinity chromatography.
- 免疫原
- Highly pure (>98%) recombinant hEGF (human EGF).
- Top Product
- Discover our top product EGF Primary Antibody
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anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibody
EGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, DB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9D7F11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 690-720), (Center) antibodyEGF 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB16555 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 609-751) antibodyEGF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 小鼠 WB, ELISA, Neut 宿主: 山羊 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) antibodyEGF 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 4-00E-11 unconjugated
anti-Epidermal Growth Factor (EGF) (AA 974-1026) antibodyEGF 适用: 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 31-53) antibodyEGF 适用: 人 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor (EGF) (AA 1-30) antibodyEGF 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: To detect hEGF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- EGF (Epidermal Growth Factor (EGF))
- 别名
- EGF (EGF 产品)
- 别名
- HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
- 背景
- Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
- 基因ID
- 1950
- NCBI登录号
- NP_001954
- UniProt
- P01133
- 途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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