(1 reference)-
- 抗原 See all BDNF 抗体
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
-
适用
- 人, 大鼠, 小鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This BDNF antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- The antibody recognises BDNF.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse and Rat.
- 纯化方法
- Affinity Chromatography employing immobilized Human BDNF matrix
- 免疫原
- Highly purified (>98%) E.coli derived recombinant Human BDNF (Cat.-No PA047)
- Top Product
- Discover our top product BDNF Primary Antibody
-
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibody
BDNF 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 19-248) antibodyBDNF 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 3D9C5 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 151-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibodyBDNF 适用: Pig WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) antibodyBDNF 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (Internal Region) antibodyVerified BDNF 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236) antibodyBDNF 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-247) antibodyBDNF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- ELISA: Indirect To detect hBDNF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBDNF. Sandwich To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated anti-Human BDNF (PP1000B1 / PP1000B2) as a detection antibody,allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF. Western blot: To detect hBDNF by Western blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. The detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
- 实验流程
- NEUROTROPHIN IMMUNOHISTOCHEMISTRYThe neurotrophins have proved difficult to localize which may be due to masking by, forexample, their association with the trk receptors or very low concentrations. Wheresuccess has been achieved, the conditions required vary greatly for different tissues. Aprotocol has been included for central nervous system tissue and, while it is similar tocommonly used methods, it is important to give strict attention to details such as thoroughwashing, fixative and detergent concentrations, concentration and quality of the primaryantibodies and length of incubations, etc. You may find it possible to use alternateprotocols, however we have experienced many failures using variations of the currentprotocol (and some failures when strict adherence to the procedure is maintained). Asuitable procedure to stain nerve terminals is still being developed. Neurotrophinreceptors have proved much easier to localize. It is recommended that you include a few sections of adult rat cerebellum, spinal cord orkidney in each experiment since these are tissues which are the easiest to stain. Protocol for Immunohistochemistry in the Central Nervous System(At all steps thorough washing is necessary to reduce background)Fixation: Animals are perfused with 1% sodium nitrite in phosphate buffered saline (PBS) (about 50ml) followed by 1 liter of Zamboni's fixative (4% formaldehyde, 15% picric acid in 0. 1Mphosphate buffer). Post fix for no longer than 2 hours. Tissue Preparation: Tissues are removed and washed briefly with PBS followed by cryoprotection in 30%sucrose in PBS overnight at 4°C. 30 µm cryostat sections are cut and washed with agitationin: PBS (1x15min)50% ethanol (3x15min)
- 限制
- 仅限研究用
-
- 溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2, without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, (2009) (PubMed).
-
: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, (2009) (PubMed).
-
- 抗原
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- 别名
- BDNF (BDNF 产品)
- 别名
- ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody
- 背景
- Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor
- 基因ID
- 627
- NCBI登录号
- NP_001137277
- UniProt
- P23560
- 途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
-
You are here:
