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Ki-67 抗体

MKI67 适用: 人 IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN115328
发货至: 中国
  • 抗原 See all Ki-67 (MKI67) 抗体
    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
    适用
    • 196
    • 86
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Ki-67 antibody is un-conjugated
    应用范围
    • 86
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    • 50
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    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    特异性
    This antibody reacts with a nuclear antigen present in proliferating human cells. It can be used to evaluate the ki-67 labeling index in various tumors.
    Cellular Localization: Nuclear.
    纯化方法
    Purified
    免疫原
    Synthetic peptide from 62 base pair region of the human Ki-67 antigen.
    Top Product
    Discover our top product MKI67 Primary Antibody
  • 应用备注
    Immunohistochemsistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
    别名
    Ki-67 (MKI67 产品)
    别名
    MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody
    背景
    Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.Synonyms: Ki67 antigen, MKI67, Proliferation marker
    基因ID
    4288
    NCBI登录号
    NP_001139438
    UniProt
    P46013
    途径
    Glycosaminoglycan Metabolic Process
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