兔 anti-人 IgG Antibody

ABIN115322 产品详细信息, 供应商: Log in to see
抗原
适用

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宿主

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克隆类型
多克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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免疫原 IgG isolated from a pool of normal Human sera.
纯化方法 Purified
背景 IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75 % of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66 %), IgG2 (23 %), IgG3 (7 %) and IgG4 (4 %).Synonyms: Human Immunoglobulin G
研究领域 Immunology, Secondary Antibodies
应用备注 Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffinembedded tissue sections. Recommended Positive Control: Tonsil

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 18.9 mg/mL
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
厂商提供的图像
Immunohistochemistry (IHC) image for Rabbit anti-Human IgG antibody (ABIN115322) Formalin fixed paraffin embedded human tonsil stained with IgG antibody. ABIN115322
背景 Koslov, Maupin, Pradhan, Morrow, Rimm: "Alpha-catenin can form asymmetric homodimeric complexes and/or heterodimeric complexes with beta-catenin." in: The Journal of biological chemistry, Vol. 272, Issue 43, pp. 27301-6, 1997 (PubMed).