E2F4 抗体 (E2F Transcription Factor 4, P107/p130-Binding)

Details for Product anti-E2F4 Antibody No. ABIN115206, 供应商: Log in to see
抗原
  • E2F4
  • e2f-4
  • 2010111M04Rik
  • AI427446
  • E2F-4
  • cb735
  • zgc:111879
  • E2F transcription factor 4, p107/p130-binding
  • E2F transcription factor 4
  • E2F transcription factor 5
  • E2F4
  • e2f4
  • E2f4
  • e2f5
适用
人, 大鼠
92
41
37
8
7
6
6
6
4
3
3
2
1
宿主
小鼠
45
29
21
克隆类型 (克隆位点)
单克隆 ()
标记
This E2F4 antibody is un-conjugated
5
4
3
3
3
3
应用范围
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
83
49
22
18
15
10
5
3
2
选项
Supplier
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Supplier Product No.
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免疫原 BALB/C mice were immunized with a recombinant human E2F-4 protein.
克隆位点 4E2F04
亚型 IgG1
特异性 This antibody reacts with a protein of 56 kDa, E2F4.
Cellular Localization: Nuclear, cytoplasmic.
纯化方法 Protein G purified ascites
别名 E2F4 (E2F4 Antibody 摘要)
背景 E2F's are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.Synonyms: E2F transcription factor 4, E2F-4, Transcription factor E2F4, p107/p130-binding
基因ID 1874
NCBI登录号 NP_001941
UniProt Q16254
研究领域 Stem Cells, Transcription Factors
途径 Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
应用备注 Immunofluorescence. Immunohistochemistry on paraffin embedded sections: 1: 25-1: 50 inan ABC method 30 minutes at room temperature. Formalin fixed paraffin embedded tissuesections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0prior to immunostaining. Western Blot (1: 50 - 1: 100). Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
缓冲液 PBS, containing sodium azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
厂商提供的图像
Immunohistochemistry (IHC) image for anti-E2F Transcription Factor 4, P107/p130-Binding (E2F4) antibody (ABIN115206) Formalin-fixed, paraffin-embedded human tonsil stained with E2F-4 Ab using peroxidase...
背景 Ikeda, Jakoi, Nevins: "A unique role for the Rb protein in controlling E2F accumulation during cell growth and differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3215-20, 1996 (PubMed).

Johnson, Cress, Jakoi, Nevins: "Oncogenic capacity of the E2F1 gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 26, pp. 12823-7, 1995 (PubMed).

Krek, Livingston, Shirodkar: "Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members." in: Science (New York, N.Y.), Vol. 262, Issue 5139, pp. 1557-60, 1993 (PubMed).

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