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小鼠 anti-人 Ig (Light Chain), (Chain kappa) Antibody

ABIN114482 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Light Chain, Chain kappa
545
399
290
66
25
16
13
10
7
6
5
3
2
2
1
适用

660
216
74
30
19
15
10
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
宿主
小鼠
405
367
158
81
21
7
4
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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免疫原 Purified Bence Jones kappa light chain protein
克隆位点 KP-53
亚型 IgG2b
纯化方法 Supernatant
别名 Immunoglobulin
背景 Immunoglobulins belong to a group of related glyco proteins which make up 20 % of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit, while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
研究领域 Secondary Antibodies, Virology
应用备注 Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in anABC method (30 minutes at room temperature). Although not required, enzymatic predigestion may help in some cases. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
背景 Hernandez, Frierson, Moskaluk, Li, Clegg, Cote, McCusker, Hankey, Edwards, Goodman: "CK20 and CK7 protein expression in colorectal cancer: demonstration of the utility of a population-based tissue microarray." in: Human pathology, Vol. 36, Issue 3, pp. 275-81, 2005 (PubMed).

Baars, De Ruijter, Smedts, Van Niekerk, Poels, Seldenrijk, Ramaekers: "The applicability of a keratin 7 monoclonal antibody in routinely Papanicolaou-stained cytologic specimens for the differential diagnosis of carcinomas." in: American journal of clinical pathology, Vol. 101, Issue 3, pp. 257-61, 1994 (PubMed).