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MHC Class I H-2Dd 抗体 (FITC)

ABIN114288 产品详细信息, 供应商: Log in to see
抗原
适用
小鼠
21
宿主
小鼠
21
克隆类型 (克隆位点)
单克隆 ()
标记
FITC
4
3
2
应用范围
Flow Cytometry (FACS)
17
10
9
3
1
Supplier
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免疫原 Recipient: C3H/HeJ Immunocyte Donor: B6 x DBA/2 spleen cells Fusion Partner: SP2/0.Ag14
克隆位点 34-5-8S
亚型 IgG2a
特异性 This anti-mouse H-2Dd monoclonal antibody is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate cells bearing the H-2Dd(H-2.4) antigen from the appropriate strains of mice.
纯化方法 Protein G Chromatography
别名 MHC Class I H-2 Dd
背景 Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1
基因ID 100045864
NCBI登录号 XP_003086970
UniProt P01900
应用备注 Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS:
限制 仅限研究用
浓度 0.1 mg/mL
缓冲液 PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Flow Cytometry (FACS) image for anti-MHC Class I H-2Dd 抗体 (FITC) (ABIN114288) FIGURE1: LFL1 - Cell Source: Spleen - Percentage of Cells Stained Above Control: 97.9%
背景 Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).