ITGA4 抗体
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- 抗原 See all ITGA4 抗体
- ITGA4 (Integrin alpha 4 (ITGA4))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This ITGA4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
- 特异性
- This antibody reacts with alpha 4 integrin.
- 纯化方法
- Protein G affinity purified immunoglobulin fraction
- 免疫原
- Peyers Patch HEV binding lymphoma line (TK1)
- 克隆位点
- R1-2
- 亚型
- IgG2b
- Top Product
- Discover our top product ITGA4 Primary Antibody
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anti-Integrin alpha 4 (ITGA4) (Internal Region) antibody
ITGA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 551-650) antibodyITGA4 适用: 人, 小鼠, 兔 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 833-977) antibodyITGA4 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7E4C5 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 376-558) antibodyITGA4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, EIA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal R1-2 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal DATK32 unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 小鼠 FACS, IP, IHC (fro), Func 宿主: 大鼠 Monoclonal DATK32 unconjugated
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- 应用备注
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Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of ABIN114228. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS buffer with 0.02 % sodium azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- ITGA4 (Integrin alpha 4 (ITGA4))
- 别名
- CD49d / ITGA4 (ITGA4 产品)
- 别名
- CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody
- 背景
- Alpha 4 integrin, which helps to mediate cell-cell and cell-matrix interactions. It combines with beta 1 and beta 7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
- 基因ID
- 16401
- NCBI登录号
- NP_034706
- UniProt
- Q00651
- 途径
- Integrin Complex
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