Vaccina Virus 抗体 (FITC)

ABIN112853 产品详细信息, 供应商: Log in to see
抗原
适用
Vaccinia virus
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宿主

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克隆类型
多克隆
标记
FITC
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应用范围
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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免疫原 Lister Strain (mixture of virions and infected cell polypeptides).
纯化方法 Affinity Chromatography.
别名 Vaccinia Virus
物质类 Virus
背景 Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As Vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During Vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize Vaccinia virus infection and enable identification other viral and cellular proteins which participate in the Vaccinia virus entry process.
研究领域 Virology
应用备注 Flow Cytometry: 1/50. ELISA and Immunofluorescence: Direct FA staining of target antigens in a permissive tissueculture system. A starting range of 1/10-1/50 is suggested. Acetone fixation of the antigen source is recommended prior to staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
状态 Liquid
浓度 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
缓冲液 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, 10 mg/mL BSA
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
背景 Fedorko, Preuss, Fahle, Li, Fischer, Hohman, Cohen: "Comparison of methods for detection of vaccinia virus in patient specimens." in: Journal of clinical microbiology, Vol. 43, Issue 9, pp. 4602-6, 2005 (PubMed).

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