Vaccinia Virus 抗体 (FITC)
VACV
适用: Vaccinia Virus (VACV)
IF, EIA, IHC (p)
宿主: 兔
Polyclonal
FITC
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- 抗原 See all Vaccinia Virus (VACV) products
- Vaccinia Virus (VACV) (Vaccina Virus (VACV))
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适用
- Vaccinia Virus (VACV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Vaccinia Virus antibody is conjugated to FITC
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应用范围
- Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Affinity Chromatography.
- 免疫原
- Lister Strain (mixture of virions and infected cell polypeptides).
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- 应用备注
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Flow Cytometry: 1/50. ELISA and Immunofluorescence: Direct FA staining of target antigens in a permissive tissueculture system. A starting range of 1/10-1/50 is suggested. Acetone fixation of the antigen source is recommended prior to staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 4-5 mg/mL (OD280 nm, E0.1% = 1.4)
- 缓冲液
- 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, 10 mg/mL BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Vaccinia Virus (VACV) (Vaccina Virus (VACV))
- 别名
- Vaccinia Virus (VACV 产品)
- 物质类
- Virus
- 背景
- Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As Vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During Vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize Vaccinia virus infection and enable identification other viral and cellular proteins which participate in the Vaccinia virus entry process.
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